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EDUCATION
PhD in Vertebrate Zoology,
Memphis State University
Major Emphasis: Molecular genetics of fish
Dissertation Topic: Sex determination in channel catfish
MS in Zoology, Idaho
State
University
Major Emphasis: Fish biology
Thesis Topic: Vomiting mechanisms in trout
BS in Biology,
Rochester Institute of Technology
Major Emphasis: Physiology
and pharmacology
COURSES TAUGHT
Undergraduate
RNR 4002 - Fisheries Literature and Communication
RNR 4061 - Independent Study
Graduate
RNR 7027 - Genetics and Culture of Finfish
RNR 7029 - Advanced Topics in Fisheries
Aquaculture Genetics
Commercial Aquaculture
Cryobiology of Aquatic Species
Cryopreservation in Aquatic Species
Flow Cytometry Applications
International Perspectives in Sustainable
Aquaculture
Politics of Water Use
Transgenic Aquatic Organisms
Impacts of Exotic Species
RNR 7070 - Fisheries Seminar
Globalization Effects on Aquaculture
Science Ethics
RNR 8000 - Thesis Research
RNR 8900 - Independent Study
RNR 9000 - Dissertation Research
RESEARCH INTERESTS
My basic area of research interest concerns the genetic improvement of aquatic
organisms for aquaculture, and the preservation of germplasm resources. This
works integrates technology from a number of areas:
1. Gene transfer and genetic engineering
Production of transgenic organisms (channel catfish, oysters and koi carp)
Development of gene transfer techniques in gametes, embryos and adult
organisms
2. Genome analysis
Molecular genetic characterization of genes (DNA sequencing, PCR)
Chromosome analysis (karyotyping and cytogenetics)
Gene mapping (fluorescent in-situ hybridization and in-situ PCR)
3. Cryopreservation
Development of techniques for frozen storage of gametes and embryos
Establishment of germplasm repositories
Conservation of genetic resources of endangered species
4. Reproductive biology, selective breeding and genetic manipulation
Development of techniques for artificial spawning and hatchery improvement
Chromosome set manipulation (polyploidy, androgenesis and gynogenesis)
Hybridization and crossbreeding
Hormone-induced sex reversal
Induction of reproductive sterility
RESEARCH IN PROGRESS
SELECTED PUBLICATIONS
Pittman-Cooley L and Tiersch
TR. 2000. Cryopreserved fish sperm as a source of DNA for genetic analysis.
North American Journal of Aquaculture, in press.
Zhang Q, Cooper RK and Tiersch
TR. 2000. Chromosomal location of the 28S ribosomal RNA gene of channel
catfish by FISH and in-situ PCR. Journal of Fish Biology, in press.
Lee N, Lambremont EN and
Tiersch TR. 2000. Dosage considerations for gamma irradiation of aquatic
species. North American Journal of Aquaculture, in press.
Zhang Q, Cooper RC and Tiersch TR. 2000. An overview of the ictalurid genome
with respect to nuclear DNA content, diploid chromosome features and physical
mapping of genes. Proceedings of the International Ictalurid Symposium,
American Fisheries Society, in press.
Segovia M,
Jenkins JA, Paniagua-Chavez P and Tiersch TR. 2000. Flow cytometric evaluation
of antibiotic effects on viability and mitochondrial function of refrigerated
sperm of Nile tilapia. Theriogeneology, in press.
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