Links : Louisiana State University  | School of Renewable Natural Resources


 

ZHIJUN LIU, Ph.D.

Professor

Medicinal Plant Research Lab















 

 

  CONTACT DETAILS

 

  Zhijun Liu, Ph.D.

  School of Renewable Natural Resources

  Louisiana State University

  Baton Rouge, LA 70803 USA

  Phone: (225) 578-4214

  Fax: (225) 578-4227                                          

  E-mail: zhiliu@lsu.edu

 

Education

 

B.S. - Henan Agricultural University, China 1982

M.S. - Oklahoma State University, USA 1988

Ph.D. - Michigan State University, USA 1991
 

Research Interests

Fingerprinting analysis and standardization protocol development of botanical extracts; bioassays-guided fractionation, isolation, purification, and identification of natural anti-angiogenic and anti-cancer compounds; preparation of botanical extracts and pure natural compounds to support pilot-scale proof of concept human studies in the treatment of cancer, obesity, diabetic retinopathy and other serious human and animal diseases related to angiogenesis; solubility improvement of pharmaceutical and bioactive compounds; and biological sample analyses using LC-MS for compounds absorption.
 

 

Grants (since 2004)

 

Liu, Zhijun. 9/15/08 – 6/30/10. Co-PI (PI: Peiying Yang, MDACC).  Sweet gum extract and late stage prostate cancer. National Center for Complementary and Alternative Medicine, NIH (Grant No.: 1R21AT004514)

Liu, Zhijun. 8/1/07 – 7/31/09. Principal Investigator.  Antiangiogenic properties of sweet leaf tea extract. National Center for Complementary and Alternative Medicine, NIH (Grant No.: R21AT002882).

Liu, Zhijun. 6/1/04-3/31/05. Principal Investigator. Preparation of Super Noni extract for biomedical evaluation.  Subcontract to LSU Health Sciences Center in New Orleans.

Liu, Zhijun. 6/1/05-11/30/05. Principal Investigator. Isolation of anticancer compound oleandrin from oleander plants. Phoenix Biotechnology, Inc.

Liu, Zhijun. 4/01/2005-3/31/2010. Co-PI. Botanicals and Metabolic Syndrome. Center Grant, National Center for Complementary and Alternative Medicine, NIH (Grant No. P50AT002776). $7,500,000 (Jianping Ye and Will Cefalu, PIs, LSU Pennington Biomedical Research Center).

 

Liu, Zhijun and Frank L. Greenway. 2004-2005. Principal Investigator. Isolation of Lipolytic Compounds from Herbs. Botanical Research Center, Functional Foods Division, Pennington Biomedical Research Center).

 

Liu, Zhijun, and Eugene A. Woltering. 2004. Principal Investigator. Black raspberry extract inhibits cancer growth by inhibiting angiogenesisLSU AgCenter Functional Foods Research Program.

 

Liu, Zhijun. 2004-2005. Principal Investigator. Fractionate, standardize, and chemically identify active compounds in foods that inhibit angiogenesis in fat tissue. Subcontract to the Community Foundation of Southeastern Michigan (Original project director: Frank L. Greenway, Pennington Biomedical Research Center).

 

Liu, Zhijun, and Eugene A. Woltering. 2004. Principal Investigator. Development of a botanical drug product for angiogenesis targeted cancer treatment.  Greater New Orleans, Inc. and Louisiana Technology Council Proof of Concept Fund.

 
Publications (past five years)

 

REFEREED

Guixin Chou, Shun-Jun Xu, Dong Liu, Gar Yee Koh, Jian Zhang, and Zhijun Liu. 2009. Quantitative and fingerprint analyses of Chinese sweet tea plant (Rubus Suavissimus S. Lee). Journal of Agricultural and Food Chemistry. Published electronically ahead of print.

David E. Wedge, Nurhayat Tabanca, Blaire J. Sampson, Christopher Werle, Betul Demerci, K. Husnu Can Baser, Peng Nan, Jia Duan, Zhijun Liu. 2009. Antifungal and Insecticidal Activity of two Juniperus Essential Oils. Natural Product Communications. 4(0): 1-5.

Wedge, David; Klun, Jerome; Tabanca, Nurhayat; Demirci, Betul; Ozek, Temel; Baser, K. Husnu; Liu, Zhijun; Zhang, Sui; Cantrell, Charles; Zhang, Jian. Bioactivity-guided Fractionation and GC-MS Fingerprinting of Angelica sinensis and A. archangelica Root Components for Antifungal and Mosquito Deterrent Activity. Journal of Agricultural and Food Chemistry (in press).

Zhijun Liu. 2008. Preparation of botanical samples for biomedical research. In the "Hot Topics" issue on “Botanical Treatments for Diabetes and Obesity”. The Journal “Endocrine, Metabolic & Immune Disorders - Drug Targets” (EMID-DT).

Yin, Jun, Aamir Zuberi, Zhanguo Gao, Dong Liu, Zhijun Liu, William T. Cefalu, Jianping Ye. 2008. Effect of Shilianhua extract and its fractions on body weight of obese mice. Metabolism Clinical and Experimental 57 (Suppl 1): S47–S51.

William T Cefalu, Jianping Ye, Aamir Zuberi, David Ribnicky, Ilya Raskin, Zhijun Liu, Zhong Q Wang, Phillip J Brantley, Luke Howard and Michael Lefevre. 2008. Botanicals and metabolic syndrome. Am J Clin Nutr 87(suppl): 481S-7S.

Yin, Jun; Gao, Zhanguo; Liu, Dong; Liu, Zhijun; Ye, Jianping. 2008. Berberine improves glucose metabolism through induction of glycolysis. Am J Physiol Endocrinol Metab 294: E148-E156.

Liu, Dong, Joshua Schwimer, Zhijun Liu, Eugene Woltering, and Frank L. Greenway. 2008. Antiangiogenic effect of curcumin in pure versus in extract forms. Pharmaceutical Biology 46: 677-682. 

David A. York, Sonyja Thomas, Frank L. Greenway, Zhijun Liu, Jennifer C. Rood. 2007. The Effect of NT, an Herbal Supplement, on Body Weight in Rats. Chinese Medicine 2:10.

Greenway, Frank L., Zhijun Liu, Ying Yu, Mary K. Caruso, Andrew Roberts, Joshua Schwimer, Alok K. Gupta, Drake Bellanger, Thomas Guillot, Eugene A. Woltering. 2007. An Assay to Measure Angiogenesis in Human Fat Tissue. Obesity Surgery 17: 510-515.

Oner, F. Hakan, Yanno Bezerra, Gholam A. Peyman, Mandi D. Conway, James M. Lewis, Zhijun Liu, Frank Greenway, Eugene A. Woltering. 2007.  Antiangiogenetic Effect of a Chinese Sweet Leaf Tea Extract in Experimental Corneal Neovascularization. Pharmaceutical Biology 45(1): 44-47.

Andrew T. Roberts, Corby K. Martin, Zhijun Liu, Ronald J. Amen, Eugene A. Woltering, Jennifer C. Rood, Mary K. Caruso, Ying Yu, Hui Xie, Frank L. Greenway.  2007. The safety and efficacy of a dietary herbal supplement and gallic acid for weight loss. Journal of Medicinal Food 10(1): 184-188.

Zhang, Jian,Ying Yu, Dong Liu, and Zhijun Liu.  2007. Extraction and composition of three naturally occurring anti-cancer alkaloids in Camptotheca acuminata seed and leaf extracts.  Phytomedicine 14: 50-56.

Zhu, Bin, Qingbiao Wang, Esben F. Roge, Peng Nan, Zhijun Liu, Yang Zhong.  2006.  Chemical variation in leaf oils of Pistacia chinensis from five locations in China.  Chemistry of Natural Products. 42(4): 422-425.

Liu, Zhijun, Joshua Schwimer, Dong Liu, James Lewis, Frank L. Greenway, David A. York, Eugene A. Woltering. 2006. Gallic acid is partially responsible for the anti-angiogenic activities of Rubus leaf extract. Phytotherapy Research 20(9): 806-813.

Frank L. Greenway, Zhijun Liu, Corby K. Martin, Wei Kai-yuan, James Nofziger, Jennifer C. Rood, Ying Yu, and Ronald J. Amen.  2006. Safety and Efficacy of NT, an Herbal Supplement, in Treating Human Obesity. International Journal of Obesity April 25: 1-5.

Liu, Zhijun, Joshua Schwimer, Dong Liu, James Lewis, Frank L. Greenway, David A. York, Eugene A. Woltering. 2006. Gallic acid is partially responsible for the anti-angiogenic activities of Rubus leaf extract. Phytotherapy Research (in press).

Zhang, Jian,Ying Yu, Dong Liu, and Zhijun Liu.  2006. Extraction and composition of three naturally occurring anti-cancer alkaloids in Camptotheca acuminata seed and leaf extracts.  Phytomedicine (in press).

1.      Zhao, Jiayuan, Jimei Liu, Xiaoyan Zhang, Zhijun Liu, Tashi Tsering, Yang Zhong and Peng Nan.  2006.  Chemical Composition of the Essential Oils of Three Wild Bergenia Species from Western China.  Flavour and Fragrance Journal 21: 431-434.

2.      Liu, Jimei, Peng Nan, Qiong Tsering, Tashi Tsering, Zhankui Bai, Li Wang, Zhijun Liu, and Yang Zhong.  2006.  Volatile constituents of the leaves and flowers of Salvia przewalskii Maxim. from Tibet.  Flavour and Fragrance Journal 21: 435-438.

3.      Frank L. Greenway, Zhijun Liu, Corby K. Martin, Wei Kai-yuan, James Nofziger, Jennifer C. Rood, Ying Yu, and Ronald J. Amen.  2006. Safety and Efficacy of NT, an Herbal Supplement, in Treating Human Obesity. International Journal of Obesity 1-5.

5.      Qiong WU, Da-Xu FU, Ai-Jun HOU, Guang-Qing LEI, Zhijun Liu, Jia-Kuan CHEN, and Tong-Shui ZHOU. 2005. Antioxidative Phenols and Phenolic Glycosides from Curculigo orchioides. Chem. Pharm. Bull. 53(8): 1065-1067.

6.      Liu, Dong, Zhanguo Gao, Jian Zhang, Jianping Ye, and Zhijun Liu. 2005. Bioassay-guided fractionation of the Rubus suavissimus leaf extracts possessing NF-Kappa-B inhibitory activities and a separable cytotoxicity. Pharmaceutical Biology 43(8): 713-717.

7.      Wang, Qingbiao, Yong Yang, Xiaomin Zhao, Bin Zhu, Peng Nan, Jiayuan Zhao, Li Wang, Fan Chen, Zhijun Liu, and Yang Zhong.  2005. Chemical variation in the essential oil of Ephedra sinica from Northeastern China.  Food Chemistry 98: 52-58.

8.      Liu, Zhijun, Joshua Schwimer, Dong Liu, Frank L. Greenway, Catherine T. Anthony, and Eugene A. Woltering.  2005. Black Raspberry Extract and Fractions Contain Angiogenesis Inhibitors.  Journal of Agriculture and Food Chemistry 53(10):3909-3915.

9.      Z Li, Z Liu. 2005. Plant regeneration from leaf petiole in Camptotheca acuminata. In Vitro Cellular and Developmental Biology – Plant 41(3):262-265.

10.  Lang, Cynthia, Zhijun Liu, H.Wayne Taylor, and David G. Baker. 2005. Effect of Eucommia ulmoides on Systolic Blood Pressure in the Spontaneous Hypertensive Rat. American Journal of Chinese Medicine 33(2): 215-230.

11.  Li, Zhanhai and Zhijun Liu.  2005.  Camptothecin production in Camptotheca acuminata hydroponic culture and nitrogen enrichments.  Canadian Journal of Plant Science 85: 447-452.

12.  Li, Zhanhai and Zhijun Liu.  2004.  Camptothecin Accumulation in Camptotheca acuminata Seedlings in Response to Acetylsalicylic Acid Treatment.  Canadian Journal of Plant Science 84:885-889.

NON-REFEREED

1.      Peiying Yang, Zhijun Liu, Carrie Cartwright, Mary V., Diana Chan, Jibin Ding, Dong Liu, and Robert A. Newman. The anti-angiogenic activity of sweet leaf tea extract is mediated by the down-regulation of bFGF and VEGF receptors. Abstract accepted for the American Association for Cancer Research annual meeting April 18 to 22, 2009 in Denver, CO.

Zhijun Liu and Peiying Yang. Novel aqueous solutions of taxol and camptothecin that retain potent cytotoxicities against human cancer cells. Abstract presented at the 4th Modern Drug Discovery & Development Summit, October 15-17, San Diego, CA.

Zhijun Liu, Dong Liu, Guixin Chou, Shunjun Xu, Aamir Zuberi, Jianping Ye. Variations in gingerols and chromatographic fingerprints of ginger root extracts from different preparation methods may account for bioactivity discrepancies among investigations. Abstract accepted to the 49th Annual Meeting of the American Society of Pharmacognosy in Athens, Greece, August 3 to 9, 2008, and published in Planta Medica, 74(9): 1110.

Pawlus, AD, Cartwright CA, Vijjeswarapu M, Liu Z, Woltering E, Newman RA. Anti-angiogenic activity from the fruit latex of Ficus carica (Fig). Abstract accepted to the 49th Annual Meeting of the American Society of Pharmacognosy in Athens, Greece, August 3 to 9, 2008, and published in Planta Medica, 74(9): 963.

Gar Yee Koh, Guixin Chou, Zhijun Liu. Purification of Chinese Sweet Leaf (Rubus suavissimus) by Alcohol Precipitation. 7th Oxford International Conference on Science of Botanicals (ICSB), April 12-15, 2008, University, Mississippi.

Zhijun Liu, Eugene A. Woltering, Dong Liu, Josh Schwimer, and Charles Johnson, and Frank L. Greenway. Persimmon calyx extract is antiangiogenic. Abstract accepted to the 48th Annual Meeting of the American Society of Pharmacognosy in Portland, Maine, July 14 to 18, 2007.

Zhijun Liu, Frank L. Greenway, Eugene A. Woltering, Dong Liu, Ying Yu, Josh Schwimer, Peng Nan, and Yang Zhong. Rhodiola crenulata root extract contains potent antiangiogenic components beyond gallic acid. Abstract accepted to the 48th Annual Meeting of the American Society of Pharmacognosy in Portland, Maine, July 14 to 18, 2007.

Xiaojun Wu, Dong Liu, and Zhijun Liu. Influence of Various Culture Conditions on the Growth of Eucommia ulmoides Hairy Roots. Abstract accepted to the 48th Annual Meeting of the American Society of Pharmacognosy in Portland, Maine, July 14 to 18, 2007.

Peiying Yang, Zhijun Liu, Carrie Cartwright, Diana Chan, Dong Liu, Jibin Ding, Robert A. Newman. Sweet gum inhibits the proliferation of prostate cancer through dual inhibition of pi3k/akt and mTOR pathways. Abstract accepted to the 48th Annual Meeting of the American Society of Pharmacognosy in Portland, Maine, July 14 to 18, 2007.

David E. Wedge, Nurhayat Tabanca, Betul Demirci, K. Husnu Can Baser, Peng Nan, Jian Duan and Zhijun Liu. The Chemical Composition and A TLC Bioautography Assay for the Detection of Antifungal Compounds from Two Juniperus Oils. 6th Oxford International Conference on Science of Botanicals (ICSB) “Quality, Safety and Processing of Botanical Products”, April 30 – May 3, 2007, University, Mississippi.

Jun Yin, Zhanguo Gao, Zhijun Liu, and Jianping Ye.  Insulin-independent Glucose-lowering Effect of SLH in vitro. Abstract accepted at the American Diabetes Association 66th Scientific Sessions (Abstract Number: 951992).

2.      Liu, Zhijun, and Eugene Woltering.  Multiple angiogenic inhibitors in a single leaf extract. Proceedings of the 2006 Miami Nature Biotechnology Winter Symposium, February 4-8, 2006, Miami Beach, Florida.

3.      Xiaojun Wu, Dong Liu, Zhijun Liu. Establishment of a hairy root culture system for producing anti-hypertensive Eucommia extract. Abstract submitted for the American Society of Pharmacognosy meeting, Corvallis Oregon, July 22-27, 2005.

5.      Liu, Dong, Joshua Schwimer, Eugene Woltering, Zhijun Liu. Antiangiogenic effect of curcumin in pure versus in extract forms. Abstract submitted for the American Society of Pharmacognosy meeting, Corvallis Oregon, July 22-27, 2005.

6.      Andrew T. Roberts, Mary K. Caruso, Ying Yu, Eugene A. Woltering, Zhijun Liu, Joshua E. Schwimer, Drake E. Bellanger, Thomas S. Guillot, and Frank L. Greenway. Validation of angiogenesis assay based on human fat tissue. Abstract at the NAASO's 2005 Annual Scientific Meeting.

7.      Liu, Zhijun, Conrad Hornick, and Eugene Woltering.  2005.  Noni tree: potential cancer preventative, therapy. Louisiana Agriculture 48 (1): 8-9.

8.      Liu, Zhijun.  2004.  Biomedical research shows tree bark reduces hypertension. Louisiana Agriculture 47 (1): 18-19.

Course taught

Fall 2007 RNR 7029 Section 1
Botanical Drug Research and Development: An Overview

This series of lectures is designed to introduce graduate students and researchers to botanical drug research and development.  This course will attempt to bridge empirical botanical knowledge and modern scientific advancement, to serve as a lightning rod for innovation, and to motivate the establishment of solid multidisciplinary collaboration essential to successful R&D of botanical drug products. 

Dr. Zhijun Liu will chair the lecture series. He is Professor of Medicinal Plants Research in the School of Renewable Natural Resources, LSU Agricultural Center. He has been conducting medicinal plant research for over 10 years and has experience in the discovery, isolation of bioactive components, standardization of botanical extracts, and technology creation. He is involved in the discovery of botanical antiangiogenic agents and creation of “Super Noni” technology that has been licensed. For more questions, please contact him at 578-4214; zhiliu@lsu.edu.

The course is designed for graduate students but may be of interest to researchers involved in botanical drug discovery and to business leaders in startup pharmaceutical/nutraceutical companies.  Senior undergraduates who are interested should contact the instructor personally.

Interested students are encouraged to enroll as soon as possible but no later than August 28 or the class may be canceled for not having required number of students.

Course information: Course number is RNR 7029, Section 1. Two Credit Hours. Time of lecture is to be arranged and once a week lecture is planned. Tentative lecture location is 225 Renewable Natural Resources Building. One mid-term exam (40%) based on lectures and handouts, and a term paper (60%) as bases of grading.  The instructor will discuss the term paper issue. 

TENTATIVE LECTURE TOPICS AND SCHEDULE IN FALL 2007:

Lecture 1         From ancient knowledge to modern medicine: what happened?

Lecture 2         Source of modern medicine: what plants have to offer?  

Lecture 3         Introduction to major medicinal constituents in plants.

Lecture 4         Look-alike plants can be vastly different in medicinal compounds. 

Lecture 5         How to prepare botanical samples for bioactivity testing.

Lecture 6         Bioactivity screening of botanical samples: in vitro and in vivo models.

Lecture 7         Unlocking the mystery of bioactive plant extracts: practical methods in isolation, purification, and identification of active molecules. 

Lecture 8         Botanical lead compounds: what do to with them? 

Lecture 9         Abandon or keep going? Truth about botanical extracts as candidate drugs.

Lecture 10       Soliciting eye-catching technology through proof of concept research.

Lecture 11       Multiplicity of botanical products and various marketing channels: FDA regulations of DSHEA and Botanical Drug Product Guidelines.

Lecture 12       Intellectual property and technology transfer issues that researchers need to know. 

Other Links

 

Disclaimer: The statements included this website, are those of Dr. Zhijun Liu only. Any statement or opinions included in these pages are not those of the LSU Agricultural Center, Louisiana State University,  the LSU Board of Supervisors, or the School of Renewable Natural Resources. Copyright © 2006.